ORIGINAL ARTICLES

Study on Cerebral Cortex Activity Induced by 40Hz Light Flicker in College Students

  • WANG Yingtong ,
  • MA Qi
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  • 1Research Center for College Student Mental Health Education, Soochow University, Suzhou 215000, China;
    2School of Education, Soochow University, Suzhou 215000, China
* MA Qi,mxqiflc@163.com

Online published: 2025-03-20

Abstract

Objective: To study the effect of 40 Hz light flicker stimulation on cerebral cortex activity and to explore a potential treatment option for cognitive neurological disorders.
Methods: A contrast threshold elevation aftereffect (TEAE) experiment was conducted on 19 undergraduate and graduate students at Soochow University. The experimental group was trained with 40 Hz light flicker stimulation, while the control group was trained with 30 Hz light flicker stimulation. Observe and compare the size of TEAE, as well as the hit rate and reaction time during the training process.
Results: The TEAE of experimental group significantly decreased after training (p<0.05), while the control group did not change significantly after training. However, the difference in the amount of change in TEAE between experimental and control groups before and after the training was not statistically significant.
Conclusion: These results indicated that 40 Hz light flicker may have a tendency to induce the human primary visual cortex activity and modulate gamma oscillations, but it was not clearly demonstrated in the current experimental context. The application of 40 Hz light flicker in the regulation of human cortical activity needs deeper and further research.

Cite this article

WANG Yingtong , MA Qi . Study on Cerebral Cortex Activity Induced by 40Hz Light Flicker in College Students[J]. Psychological Communications, 2024 , 7(3) : 168 -175 . DOI: 10.12100/j.issn.2096-5494.224022

References

[1] 刘春雷, 周仁来. (2012). 工作记忆训练对认知功能和大脑神经系统的影响. 心理科学进展, 20(7), 1003-1011. doi: 10.3724/SP.J.1042.2012.01003
[2] 魏萍, 周晓林. (2005). 从知觉负载理论来理解选择性注意. 心理科学进展, 13(4), 413-420. doi: 10.3969/j.issn.1671-3710. 2005.04.005
[3] 尹述飞, 陈祥展, 刘启珍, 丁舟舟, 李添, 杨伟平, 朱心怡. (2019). 老年人认知训练的神经机制研究. 生物化学与生物物理进展, 46(2), 152-161. doi:10.16476/j.pibb.2018.0147
[4] 张赫, 郑焱. (2019). β淀粉样蛋白级联假说相关的阿尔茨海默病发病机制及防治策略研究进展. 中国医学科学院学报, 41(5), 702-708. doi: 10.3881/j.issn.1000-503X.10875
[5] 张佳燚, 孙璐瑶, 曹津萌, 程宇, 李双, 刘喜富. (2024). 阿尔茨海默病的发病机制及治疗新进展. 河北师范大学学报(自然科学版), 48(4), 401-413. doi: 10.13763/j.cnki.jhebnu.nse.202404011
[6] Adaikkan C., Middleton S. J., Marco A., Pao P. C., Mathys H., Kim D. N., .. Tsai L. H. (2019). Gamma entrainment binds higher-order brain regions and offers neuroprotection. Neuron, 102(5), 929-943.e8. doi: 10.1016/j.neuron.2019.04.011
[7] Agger M. P., Horning M., Carstensen M. S., Danielsen E. R., Baandrup A. O., Nguyen M., .. Kjær T. W. (2023). Study on the effect of 40 Hz non-invasive light therapy system. A protocol for a randomized, double-blinded, placebo-controlled clinical trial. Front Aging Neurosci, 15, 1250626. doi: 10.3389/fnagi.2023.1250626
[8] Blakemore, C., & Campbell, F. W. (1969). On the existence of neurones in the human visual system selectively sensitive to the orientation and size of retinal images. J Physiol, 203, 237-260. doi: 10.1113/jphysiol.1969.sp008862
[9] Buzsáki, G., & Wang, X. (2012). Mechanisms of gamma oscillations. Annu Rev Neurosci, 35, 203-225. doi: 10.1146/annurev-neuro- 062111-150444
[10] Chan D., Suk H. J., Jackson B. L., Milman N. P., Stark D., Klerman E. B., .. Tsai L. H. (2022). Gamma frequency sensory stimulation in mild probable Alzheimer’s dementia patients: Results of feasibility and pilot studies. PloS one, 17(12), e0278412. doi: 10.1371/journal.pone.0278412
[11] Etter G., van der Veldt S., Manseau F., Zarrinkoub I., Trillaud-Doppia E., & Williams S. (2019). Optogenetic gamma stimulation rescues memory impairments in an Alzheimer’s disease mouse model. Nat Commun, 10(1), 5322. doi: 10.1038/s41467-019-13260-9
[12] Fitzgerald, P. J., & Watson, B. O. (2018). Gamma oscillations as a biomarker for major depression: An emerging topic. Transl Psychiatry, 8, 1-7. doi: 10.1038/s41398-018-0239-y
[13] Fu W., Yu X., Lai M., Li Y., Yang Y., Qin Y., .. Wang C. (2024). Gamma oscillations induced by 40-Hz visual-auditory stimulation for the treatment of acute-phase limb motor rehabilitation after stroke: Study protocol for a prospective randomized controlled trial. Trials, 25(1), 284. doi: 10.1186/s13063-024-08121-w
[14] He D., Kersten D., & Fang F. (2012). Opposite modulation of high- and low-level visual aftereffects by perceptual grouping. Curr Biol, 22(11), 1040-1045. doi: 10.1016/j.cub.2012.04.026
[15] Iaccarino H. F., Singer A. C., Martorell A. J., Rudenko A., Gao F., Gillingham T. Z., .. Tsai L. H. (2016). Gamma frequency entrainment attenuates amyloid load and modifies microglia. Nature, 540(7632), 230-235. doi: 10.1038/nature20587
[16] Ismail, R., Hansen, A. K., Parbo, P. , Brndgaard, H. , & Borghammer, P. (2018). The effect of 40-Hz light therapy on amyloid load in patients with prodromal and clinical Alzheimer’s disease. Int J Alzheimers Dis, 2018, 6852303. doi: 10.1155/2018/6852303
[17] Jannati A., Oberman L. M., Rotenberg A., & Pascual-Leone A. (2023). Assessing the mechanisms of brain plasticity by transcranial magnetic stimulation. Neuropsychopharmacology, 48(1), 191-208. doi: 10.1038/s41386-022-01453-8
[18] Jeter P. E., Dosher B. A., Liu S. H., & Lu Z. L. (2010). Specificity of perceptual learning increases with increased training. Vision Res, 50(19), 1928-1940. doi: 10.1016/j.visres.2010.06.016
[19] Johansson H., Folkerts A. K., Hammarström I., Kalbe E., & Leavy B. (2023). Effects of motor-cognitive training on dual-task performance in people with Parkinson’s disease: A systematic review and meta-analysis. J Neurol, 270(6), 2890-2907. doi: 10.1007/s00415-023-11610-8
[20] Jones M., McDermott B., Oliveira B. L., O’Brien A., Coogan D., Lang M., .. Shahzad A. (2019). Gamma band light stimulation in human case studies: Groundwork for potential Alzheimer’s disease treatment. J Alzheimers Dis, 70(1), 171-185. doi: 10.3233/JAD-190299
[21] Khachatryan E., Wittevrongel B., Reinartz M., Dauwe I., Carrette E., Meurs A., .. Van Hulle M. M. (2022). Cognitive tasks propagate the neural entrainment in response to a visual 40 Hz stimulation in humans. Front Aging Neurosci, 14, 1010765. doi: 10.3389/fnagi.2022.1010765
[22] Kim S., Jang S. K., Kim D. W., Shim M., Kim Y. W., Im C. H., & Lee S. H. (2019). Cortical volume and 40-Hz auditory-steady-state responses in patients with schizophrenia and healthy controls. NeuroImage Clin, 22, 101732. doi: 10.1016/j.nicl.2019.101732
[23] Martorell A. J., Paulson A. L., Suk H. J., Abdurrob F., Drummond G. T., Guan W., ..Tsai L. H. (2019). Multi-sensory gamma stimulation ameliorates Alzheimer’s-associated pathology and improves cognition. Cell, 177(2), 256-271.e22. doi: 10.1016/j.cell.2019.02.014
[24] Murdock M. H., Yang C. Y., Sun N., Pao P. C., Blanco-Duque C., Kahn M. C., .. Tsai L. H. (2024). Multisensory gamma stimulation promotes glymphatic clearance of amyloid. Nature, 627(8002), 149-156. doi: 10.1038/s41586-024-07132-6
[25] Nguyen L., Murphy K., & Andrews G. (2019). Cognitive and neural plasticity in old age: A systematic review of evidence from executive functions cognitive training. Ageing Res Rev, 53, 100912. doi: 10.1016/j.arr.2019.100912
[26] Park S. S., Park H. S., Kim C. J., Baek S. S., Park S. Y., Anderson C.,.. Kim T. W. (2022). Combined effects of aerobic exercise and 40-Hz light flicker exposure on early cognitive impairments in Alzheimer’s disease of 3×Tg mice. J Appl Physiol, 132(4), 1054-1068. doi: 10.1152/japplphysiol.00751.2021
[27] Soula M., Martín-Ávila A., Zhang Y., Dhingra A., Nitzan N., Sadowski M. J., .. Buzsáki G. (2023). Forty-hertz light stimulation does not entrain native gamma oscillations in Alzheimer’s disease model mice. Nat Neurosci, 26(4), 570-578. doi: 10.1038/s41593-023-01270-2
[28] Uchiyama Y., Sakai H., Ando T., Tachibana A., & Sadato N. (2021). BOLD signal response in primary visual cortex to flickering checkerboard increases with stimulus temporal frequency in older adults. PloS one, 16(11), e0259243. doi: 10.1371/journal.pone.0259243
[29] Wang J., Fang Y., Wang X., Yang H., Yu X., & Wang H. (2017). Enhanced gamma activity and cross-frequency interaction of resting-state electroencephalographic oscillations in patients with Alzheimer’s disease. Front Aging Neurosci, 9, 243. doi: 10.3389/fnagi.2017.00243
[30] Watson, A. B., & Pelli, D. G. (1983). QUEST: A Bayesian adaptive psychometric method. Percept Psychophys, 33(2), 113-120. doi: 10.3758/bf03202828
[31] Yang, Y. L. & Lai, T. W. (2023). Chronic visual stimulation with LED light flickering at 24, 40, or 80 Hz failed to reduce amyloid β load in the 5XFAD Alzheimer’s disease mouse model. eNeuro, 10(8), ENEURO.0189-23.2023. doi: 10.1523/ENEURO.0189-23.2023
[32] Yuasa K., Hirosawa T., Soma D., Furutani N., Kameya M., Sano M., .. Kikuchi M. (2023). Eyes-state-dependent alterations of magnetoencephalographic connectivity associated with delayed recall in Alzheimer’s disease via graph theory approach. Front Psychiatry, 14, 1272120. doi: 10.3389/fpsyt.2023.1272120
[33] Zhang Y., Zhang Z., Luo L., Tong H., Chen F., & Hou S. (2021). 40 Hz light flicker alters human brain electroencephalography microstates and complexity implicated in brain diseases. Front Neurosci, 15, 777183. doi: 10.3389/fnins.2021.777183
[34] Zheng L., Yu M., Lin R., Wang Y., Zhuo Z., Cheng N., .. Hou S. (2020). Rhythmic light flicker rescues hippocampal low gamma and protects ischemic neurons by enhancing presynaptic plasticity. Nat Commun, 11(1), 3012. doi: 10.1038/s41467-020-16826-0
[35] Zhou X., He Y., Xu T., Wu Z., Guo W., Xu X., .. Chen J. (2024). 40 Hz light flickering promotes sleep through cortical adenosine signaling. Cell Res, 34(3), 214-231. doi: 10.1038/s41422-023-00920-1
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